Genetically modified coffee plants expressing the Bacillus thuringiensis cry1Ac gene for resistance to leaf miner

Leroy Thierry, Henry A.M., Royer Monique, Altosaar Illimar, Frutos Roger, Duris Daniel, Philippe René. 2000. Genetically modified coffee plants expressing the Bacillus thuringiensis cry1Ac gene for resistance to leaf miner. Plant Cell Reports, 19 (4) : 382-389.

Autre titre : Expression du gène cry1Ac de Bacillus thuringiensis dans des caféiers génétiquement transformés pour la résistance à la mineuse des feuilles

Résumé : A synthetic version of the cry1Ac gene of Bacillus thuringiensis has been used for the transformation of coffee species (Coffea canephora and C. arabica) to confer resistance to an important pest, the coffee leaf miner (Perileucoptera coffeella and other Leucoptera spp). Somatic embryos were co-cultivated with the LBA4404 strain of Agrobacterium tumefaciens containing the cry1Ac gene. More than 100 transformed plants from independent transformation events were obtained for each coffee genotype. The integration and expression of the cry1Ac gene was studied, and effective resistance of transgenic plants against leaf miner was verified in bioassays with the insects. These plants could represent a good opportunity to analyse the impact of genetic engineering of perennial crops for sustainable resistance to an obligate endocarpic pest using a B. thuringiensis insecticidal protein.

Mots-clés Agrovoc : Coffea arabica, Coffea canephora, transformation génétique, Bacillus thuringiensis, transfert de gène, Agrobacterium tumefaciens, résistance aux facteurs nuisibles, Perileucoptera coffeella, Leucoptera, expression des gènes

Classification Agris : F30 - Génétique et amélioration des plantes
H10 - Ravageurs des plantes

Auteurs et affiliations

• Leroy Thierry, CIRAD-CP-CAFE (FRA)
• Henry A.M.
• Royer Monique, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Altosaar Illimar, University of Ottawa (CAN)
• Frutos Roger, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Duris Daniel, CIRAD-CP-CAFE (FRA)
• Philippe René, CIRAD-CP-COCOTIER (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/264021/)

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