Caruana Marie-Line, Galzi Serge, Séchet Hélène, Bousalem M., Bringaud C..
1996. Etiologie de la maladie de la mosaïque des bractées des bananiers pour un diagnostic cible.
In : LPRC, Laboratoire de Phytovirologie des Régions Chaudes, CIRAD-ORSTOM. Rapport d'activités 1994-1995. CIRAD; ORSTOM
Autre titre : Etiology of the banana bract mosaic disease : target-diagnosis
Résumé : A potyvirus was involved in the banana bract mosaïc disease in the Philippines. Its characterization went on to develop detection tools in order to control the disease. Specific monoclonal antibodies were obtained from the Philippines isolate and consistently reacted with a 30Kd protein. A direct Tissue Blot Immunoprinting Assay was also etablished to make easier the field detection. The disease was experimentally transmitted from the Philippines isolate to healthy banana plants using #Pentalonia nigronervosa#. These infected plants were considered as a viral reference of the disease. A molecular characterization of the 3' region of the Red viral genome was developed on amplified DNA fragments using potyvirus specific degenerated primers and reverse transcriptase PCR amplification
Mots-clés Agrovoc : Musa, virose, bractée, Potyvirus, Clonage moléculaire, infection expérimentale
Mots-clés géographiques Agrovoc : Philippines
Mots-clés complémentaires : Mosaïque
Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes
Auteurs et affiliations
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Caruana Marie-Line, CIRAD-FLHOR-BCPR (FRA)
ORCID: 0000-0003-4486-2449
- Galzi Serge, CIRAD-FLHOR-BEP (FRA)
- Séchet Hélène, CIRAD-FLHOR-BCPR (FRA)
- Bousalem M.
- Bringaud C.
Source : Cirad - Agritrop (https://agritrop.cirad.fr/389560/)
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