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Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings

Triques K., Rival Alain, Beulé Thierry, Dussert Stéphane, Hocher Valérie, Verdeil Jean-Luc, Hamon Serge. 1997. Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings. Plant Cell, Tissue and Organ Culture, 49 : pp. 227-231.

Journal article ; Article de revue à facteur d'impact
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Autre titre : Modifications des activités de la carboxylase dans les cultures in vitro des zygotes de cocotier : comparaison avec les activités similaires lors de la germination des semences

Abstract : Phosphoenolpyruvate Carboxylase (PEPC; EC: 4.1.1.31) and Ribulose 1.5-bisphosphate Carboxylase/Oxygenase (RubisCO; EC: 4.1.1.39) enzyme specific activities were measured during the #in vitro# development of coconut (#Cocos nucifera# L.) zygotic mature embryos into plantlets and compared with those of palms produced by conventional seed germination. At the time of initiation of germination, high PEPC and low RubisCO activities were measured in both cultured and conventionally germinated embryos, thus indicating an anaplerotic C02 fixation. During both #in vitro# and #in planta# development, RubisCO progressively took over and became the main route for inorganic carbon fixation. The #in vitro#-grown coconut plantlets showed a faster decrease in their PEPC/RubisCO ratio than the seedlings, suggesting that an earlier transition from a heterotrophic to an autotrophic mode on carbon fixation takes place in the #in vitro#-denved material. Just before acclimatization, the RubisCO activity in #in vitro#-derived plantlets (2.83 pmol C02h-1mg-1 TSP) was lower than that in seedlings (6.98 pmol C02h-1 mg-1 TSP ) for the same age. Nevertheless, after acclimatization, RubisCO activities were comparable in both #in vitro# and #in planta# germinated materiel. (Résumé d'auteur)

Mots-clés Agrovoc : Cocos nucifera, Culture d'embryon, Activité enzymatique, Phosphoenolpyruvate carboxylase, Rubisco, Nutrition des plantes, Photosynthèse

Classification Agris : F60 - Plant physiology and biochemistry

Auteurs et affiliations

  • Triques K.
  • Rival Alain, CIRAD-CP-PALMIER (FRA) ORCID: 0000-0001-6304-3268
  • Beulé Thierry, CIRAD-CP-PALMIER (FRA)
  • Dussert Stéphane
  • Hocher Valérie
  • Verdeil Jean-Luc, CIRAD-CP-COCOTIER (FRA)
  • Hamon Serge

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/389619/)

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