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A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers

Lanaud Claire, Risterucci Ange-Marie, N'Goran Jeanne A.K., Clément Didier, Flament Marie-Henriette, Laurent V.. 1995. A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers. In : Plant genome III = [Génome des plantes III]. Scherago International. s.l. : s.n., p. 56. International Conference on the Status of Plant Genome Research. 3, San Diego, États-Unis, 15 January 1995/19 January 1995.

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Autre titre : Une carte des liaisons génétiques de Theobroma cacao L. basées sur des isoenzymes et des marqueurs RFLP et RAPD

Abstract : A linkage map of cacao genome have been constructed using isoenzymes, RFLPOs, and RAPDs markers. A genomic library and cDNA library were constructed from germinated seeds of an Amelonado clone. The screening of probes was made on 5 genotypes, using 5 restriction enzymes. Among 587 tested probes, 52% of cDNA and 44% of genomic probes revealed polymorphic patterns. A population of 100 individuals derived from a cross between two heterozygous genotypes has been used. Segregation analyses were performed with JoinMap programm. The map comprised 193 loci: 5 isoenzymes, 101 cDNA/RFLPs, 4 loci from genes of known fonction, 55 genomic DNA/RFLPs and 28 RAPDs. Ten linkage groups, which putatively correspond to the 10 gametic chromosomes of cacao, have been identified. The map covers a total length of 759 cM with a 3,9 cM average distance between two markers. A small fraction (9%) of the markers deviated significantly from the expected mendelian ratios

Mots-clés Agrovoc : Theobroma cacao, ADN, Carte génétique, RAPD, RFLP, Isoenzyme, Liaison génétique

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

Source : Cirad - Agritrop (https://agritrop.cirad.fr/389712/)

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