Molecular mapping of cocoa chromosomes

Lanaud Claire, Risterucci Ange-Marie, N'Goran Jeanne A.K., Clément Didier. 1994. Molecular mapping of cocoa chromosomes. In : 4th International Congress of Plant Molecular Biology. ISPMB. Montpellier : CIRAD, 1 p. International Congress of Plant Molecular Biology. 4, Amsterdam, Pays-Bas, 19 June 1994/24 June 1994.

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Autre titre : Carte génétique du cacaoyer

Abstract : A map of cocoa genome has been constructed using isozymes, RAPD, c-DNA and genomic probes. c-DNA library were constructed from germinated seeds of an Amelonado clone.The selection of probes was made on 5 genotypes, using 5 restriction enzymes. 500 tested probes, 33 % of c-DNA and 44 % of genomic revealed polymorphic pattrns. The segregations of markers were studied on a "back-cross type"'mapping population of 100 individuals. After the analises were performed using Mapmaker V.2.0 with a lod score = 3 and 0 = 0.35, it was possible to construct map integrating close to 170 RFLP, RAPD and isozymes markers. (Résumé d'auteur)

Mots-clés Agrovoc : Theobroma, Marqueur génétique, RAPD, RFLP, Carte génétique

Classification Agris : F30 - Plant genetics and breeding

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Source : Cirad - Agritrop (

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