Improvement of Citrus somatic embryo development by temporary immersion

Cabasson Cécile, Alvard Daniel, Dambier Dominique, Ollitrault Patrick, Teisson Claude. 1997. Improvement of Citrus somatic embryo development by temporary immersion. Plant Cell, Tissue and Organ Culture, 50 (1) : pp. 33-37.

Journal article ; Article de revue à facteur d'impact
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Autre titre : Amélioration du développement des embryons somatiques des Citrus par l'immersion temporaire

Abstract : Liquid medium improves and facilitates somatic embryo development from #Citrus deliciosa# Ten. suspension cultures. Three different culture conditions were compared to determine a means of overcoming poor somatic embryo development. Somatic embryos derived from suspension cultures were plated on solid medium, maintained in suspension culture or temporarily immersed. About 60% of somatic embryos plated on solid medium developed to the cotyledonary stage, but were hyperhydric. Continuous growth in suspension culture at 100 rpm hindered cotyledon and protoderm formation, and somatic embryos were unable to develop beyond the globular stage. Temporary immersion promoted somatic embryo development, i.e. 66% of the somatic embryos produced were cotyledonary, and were morphologically similar to nucellar embryos. This latter culture system also improved regeneration synchronization by hampering secondary embryogenesis at the onset of germination. Irrespective of the culture system used, most cotyledonary somatic embryos studied had no caulinary meristem or starch and protein reserves, thus explaining the low germination rates obtained. (Résumé d'auteur)

Mots-clés Agrovoc : Citrus, Embryon somatique, Développement embryonnaire, Technique de culture, Biotechnologie végétale

Classification Agris : F62 - Plant physiology - Growth and development

Auteurs et affiliations

  • Cabasson Cécile
  • Dambier Dominique, CIRAD-FLHOR-RGAP (FRA)
  • Ollitrault Patrick, CIRAD-FLHOR-AAF (GLP)

Autres liens de la publication

Source : Cirad - Agritrop (

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