Molecular search for mRNA and genomic markers of the oil palm "mantled" somaclonal variation

Rival Alain, Tregear James, Verdeil Jean-Luc, Richaud Frédérique, Beulé Thierry, Duval Yves, Hartmann Caroline, Rode A.. 1998. Molecular search for mRNA and genomic markers of the oil palm "mantled" somaclonal variation. In : Proceedings of the First International symposium on biotechnology of tropical and subtropical species, Brisbane, Queensland, Australia, September 29-October 3, 1997. Part 2. Drew R.A. (ed.). ISHS. Louvain : ISHS [Belgique], pp. 165-171. (Acta Horticulturae, 461) ISBN 978-90-66058-90-3 International Symposium on Biotechnology of Tropical and Subtropical Species. 1, Brisbane, Australie, 29 September 1997/3 October 1997.

Paper with proceedings
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Autre titre : Recherche de marqueurs moléculaires ARNm et génomiques de la variation somaclonale "mantled" du palmier à huile

Abstract : We are attempting to identify mRNA and genomic markers for screening of the "mantled" flowering abnormality found in typically 5-10 % of regenerated plants. Analyses of genome size and structure are being performed using flow cytometry and RAPD techniques. Studies of genomic methylation, which is thought to play an important role in the induction of somaclonal variation, are under way. We are analyzing genomic DNA from somatic embryogenesis-derived material by estimating ratios using HPLC following enzymic hydrolysis. Potential mRNA markers are being identified using the differential display technique and their expression patterns are being confirmed by Northern blotting. (Résumé d'auteur)

Mots-clés Agrovoc : Elaeis guineensis, RAPD, Marqueur génétique, Nombre chromosomique, Clonage moléculaire, Génome, Technique analytique, Embryon somatique, Variation somaclonale

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

  • Rival Alain, CIRAD-CP-PALMIER (FRA) ORCID: 0000-0001-6304-3268
  • Tregear James, CIRAD-CP-Amélioration des plantes (FRA)
  • Verdeil Jean-Luc, CIRAD-CP-COCOTIER (FRA)
  • Richaud Frédérique, CIRAD-CP-PALMIER (FRA)
  • Beulé Thierry, CIRAD-CP-PALMIER (FRA)
  • Duval Yves, CIRAD-CP-PALMIER (FRA)
  • Hartmann Caroline
  • Rode A.

Autres liens de la publication

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