Towards a perennial upland rice

McNally Kenneth L., Schmit Véronique, Courtois Brigitte. 1998. Towards a perennial upland rice. . s.l. : Plant and Animal Genomes VI Conference, 10 p. Plant and Animal Genomes Conference. 6, San Diego, États-Unis, 18 January 1998/22 January 1998.

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Autre titre : Vers un riz pluvial pérenne

Abstract : In order to provide both a means of livelihood and control erosion in the uplands of Southeast Asia, a perennial upland rice is under development. Genes for rhizome expression are being introgressed from #Oryza longistaminata# (Ol) into #O. sativa# (Os). Three related mapping populations have been or are being developed: 1) a BC1F1 population of 65 individuals where the recurrent parent is Ol (BCLF1) (this population is not segregating for rhizome expression but is segregating for resistance to the nematode #Meloidogyne graminicola#, 2) a BC1F2 population (BCLF2) of 215 individuals derived from 30 BCLF1 parents segregating for both rhizome expression and nematode resistance, and 3) a BCLF1/BCLF1 intercross population in an early stage of development. For these mapping populations, the Os parent was the indica landrace BS125 while the Ol parent was the accession WLO2 for the F1 generation. These parents are identical to those used for the Cornell genomic map. Either WLO2 or SL313 were used as the Ol parent for the BC1F1 generation. Hand pollination for selfed seed, embryo rescue, and in vitro seed germination were found necessary for the production of these materials in order to overcome reproductive barriers and low seedling vigor. Currently, 29 RFLP and 5 STS markers have been screened and found to be polymorphic for the BCLF1 population. Work is underway mapping the BCLF1 population and 193 BCLF2 individuals using RFLP, STS, microsatellite markers, and candidate gene analysis for nematode resistance as well as phenotyping 30 BCLF1 and 157 BCLF2 individuals for traits associated with rhizome expression, root development, and nematode resistance. Synteny between the genomes of rice and sorghum will be used to determine if markers associated with rhizome expression in sorghum are also linked with QTL for rhizome expression in rice. (Résumé d'auteur)

Mots-clés Agrovoc : Oryza longistaminata, Oryza sativa, Riz pluvial, Plante pérenne, Amélioration des plantes, Recombinaison, Variété introduite, Résistance aux organismes nuisibles

Classification Agris : F30 - Plant genetics and breeding

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