Sardana R., Dukandjiev S., Giband Marc, Cheng X., Cowan K., Sauder C., Altosaar Illimar. 1996. Construction and rapid testing of synthetic and modified toxin gene sequences CryIA (b&c) by expression in maize endosperm culture. Plant Cell Reports (15) : 677-681.
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Résumé : The synthesis of two modified genes, CryIA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CrylA(c) gene was put under the control of a maize ubiquitin promoter. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.
Mots-clés Agrovoc : Zea mays, gène, séquence nucléotidique, PCR, endosperme, endotoxine, culture de tissu, Bacillus thuringiensis
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Sardana R.
- Dukandjiev S.
-
Giband Marc, CIRAD-CA-COTON (FRA)
ORCID: 0000-0002-5553-5614
- Cheng X.
- Cowan K.
- Sauder C.
- Altosaar Illimar
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/390159/)
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