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Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross

Luro François, Laigret Frédéric, Lorieux Mathias, Ollitrault Patrick. 1996. Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross. In : Proceedings International Society Citriculture. Volume 2. s.l. : s.n., pp. 862-866. International Citrus Congress. 8, Sun City, Afrique du Sud, 12 May 1996/17 May 1996.

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Abstract : We have started genetic mapping of the citrus genome by segregation analysis of different molecular markers (RAPDS, RFLP and isozymes) in the progeny of FAO 30573 hybrid (#Citrus reshni# x #Pancirus trifoliata#) and pummelo (#C. grandi#s) used respectively as paternal and maternal parents. A total DNA library and a cDNA library were constructed from sweet orange (#C. sinensis#) leaves of the Valencia Late clone. The screening of probes w.as made on the parent genotypes using 4 restriction enzymes. Among 85 tested probes, 85 % of cDNA probes and 10 % of genomic probes revealed polymorphic patterns. Segregation analysis w.as performed w.ith the MapMaker program. The map of the intergeneric hybrid comprised 95 foci linked in 12 groups : 7 isozymes, 45 cDNA/RFLPs, 1 rDNA/RFLPs, 40 RAPDs and 2 inter-microsatellite sequences. The map of pummelo comprised 34 markers making 7 linkage groups : 3 isozymes, 13 cDNA/RFLP, 15 RAPDs and 3 inter-microsatellite sequences. Only the first map shows evidence of skewed segregation within some linkage groups (37.5% of total markers). Some applications of citrus genetic maps are given. (Résumé d'auteur)

Mots-clés Agrovoc : Citrus, Carte génétique, Marqueur génétique, RAPD, RFLP, Isoenzyme, Ségrégation

Mots-clés complémentaires : Marqueur biochimique

Classification Agris : F30 - Plant genetics and breeding

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/390226/)

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