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Production of haploid plants and embryogenic calli of clementine (Citrus reticulum Blanco) after in situ parthenogenesis induced by irradiated pollen

Ollitrault Patrick, Allent V., Luro François. 1996. Production of haploid plants and embryogenic calli of clementine (Citrus reticulum Blanco) after in situ parthenogenesis induced by irradiated pollen. In : Proceedings International Society Citriculture. Volume 2. s.l. : s.n., pp. 913-917. International Citrus Congress. 8, Sun City, Afrique du Sud, 12 May 1996/17 May 1996.

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Abstract : An efficient method for the production of haploid plants or cell lines would open new avenues for citrus genetics and breeding. In view. of the limited success obtained with androgenesis as described in the literature, we have instead experimentally induced #in situ# parthenogenesis. Flowers of Clementines were pollinated in the field with pollen of.Meyer lemon (#C. limon#) irradiated at 0, 300, 600 and 900 Gray. The rate of #in vitro# pollen germination was not modified by irradiation up to 900 Gray. Fruit set was slightly affected only by the 900 Gray dose. However, the number per fruit and size of seeds was drastically reduced by the 300 Gray dose and higher. Embryos found in the small seeds of irradiated treatments were saved #in vitro# and the ploidy level of the plantlets obtained was evaluated by- flow cytometry. Some haploid plantlets were identified and some mixoploid structures (haploid/diploid) were observed. From embryos cultivated in a medium favourable to callogenesis two embryogenic haploid callus lines were obtained which have produced many secondary haploid embryos. Isoenzyme profiles of the haploid plants and calli studied were consistent with the theoretical profiles for haploid Clementine. The #in vitro# growth of haploid plantlets was very slow but grafting haploid shoots onto one year old #C. macrophylla# has resulted in the satisfactory development of haploid Clementine. Moreover these haploid embryogenic calli create the opportunity for triploid synthesis by 'diploid+haploid'somatic hybridisation and also have uses in genetic transformation programmes. (Résumé d'auteur)

Mots-clés Agrovoc : Citrus reticulata, Gynogénèse, Parthénogénèse, Haplométhode, Culture d'embryon, Haploïdie, Radiosensibilité

Classification Agris : F02 - Plant propagation

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/390231/)

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