Mapping of the cocoa genome using biochemical and molecular markers

Lanaud Claire, Risterucci Ange-Marie, N'Goran Jeanne A.K., Sounigo Olivier, Clément Didier. 1994. Mapping of the cocoa genome using biochemical and molecular markers. In : Seventeenth international congress of genetics : genetics and the understanding of life. Volume of abstracts. International Convention Centre. s.l. : s.n., p. 196. International Congress of Genetics. 17, Birmingham, Royaume-Uni, 15 August 1993/21 August 1993.

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Abstract : A map of the cocoa genome is being constructed using biochemical and molecular markers. A library of c-DNA probes was established using germinating beans from the clone lFC5 (Amelonado). After extraction techniques had been optimized, the selection of polymorphic probes from this library was made using a sample of six clones belonging to different genetic groups and, using five restriction enzymes, out of 162 probes tested, 45% of the probes were shown to be polymorphic for at least one restricting enzyme. EcoRI is the enzyme which showed the most polymorphic patterns. The selection and segregation of RAPD type markers were also studied; the primers used are from the OPERON kits. By using populations of the back-cross type for this mapping it was possible to construct a first map integrating RFLP markers, RAPD markers and enzyme markers. (Résumé d'auteur)

Mots-clés Agrovoc : Theobroma cacao, Carte génétique, Marqueur génétique

Classification Agris : F30 - Plant genetics and breeding

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Source : Cirad - Agritrop (

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