Zaremski Alba, Ducousso Marc, Prin Yves, Fouquet Daniel.
1997. Caractérisation moléculaire des champignons destructeurs des bois tropicaux.
In : Forest Products for Sustainable Forestry : final program and proceedings = [Produits forestiers pour une foresterie durable : programme final et débats]. IUFRO
Titre anglais : Molecular characterization of tropical wood-decaying fungi by RFLP analysis of PCR amplified rDNA
Résumé : Five brown-rot fungi (Tyromyces palustris, Gloeophyllum trabeum, Antrodia sp., Poria placenta, Coniophora puteana) and three white-rot fungi (Coriolus ve.rsicolor, Pycnoporus sanguineus, Lentinus squarrosulus) registered as representative of international standards of wood durability tests have been genetically characterized through PCR/RFLP techniques. The Internal Transcribed Spacer between 18s and 25s rRNA has been amplified using universal primers. The amplified fragments were compared by using ten restriction enzymes. The analysis of RFLP gel electrophoresis allowed to clearly discriminate all the strains from each others. These RFLP analyses constitute a range of reference profiles to further analyze the genetic diversity of the 150 wood-degrading fungi strains currently in collection in the laboratory.
Mots-clés Agrovoc : Champignon, biologie moléculaire
Mots-clés complémentaires : Pourriture brune, Pourriture fibreuse
Classification Agris : K50 - Technologie des produits forestiers
H20 - Maladies des plantes
Auteurs et affiliations
- Zaremski Alba, CIRAD-FORET-BOIS (FRA)
- Ducousso Marc, CIRAD-FORET-PLANTATIONS FORESTIERES (FRA)
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Prin Yves, CIRAD-FORET-PLANTATIONS FORESTIERES (FRA)
ORCID: 0000-0002-3706-0045
- Fouquet Daniel, CIRAD-FORET-BOIS (FRA)
Source : Cirad - Agritrop (https://agritrop.cirad.fr/391127/)
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