Le Bris M., Michaux-Ferrière Nicole, Jacob Y., Poupet A., Barthe P., Guigonis J.M., Le Page Degivry M.T.. 1999. Regulation of bud dormancy by manipulation of ABA in isolated buds of Rosa hybrida cultured in vitro. Australian Journal of Plant Physiology, 26 : 273-281.
Résumé : #In vitro# cultures showed that the proximal buds isolated from a rose ("Rosa hybrida# L. cv. Ruidriko Vivaldi(R)) stem were endodormant. Growth and a high percentage of bud break could be observed when cultures were treated with fluridone, an inhibitor of carotenoid synthesis. Flow cytometry determination of nuclear DNA content revealed that cell cycle activity of endodormant buds was arrested in the G1phase. Upon culture, the large decrease in bud ABA content was responsible for the progress from G1 to G2 phase whatever the culture medium. However, in control culture, neither cell division nor leaf primordium initiation could be observed and cells appeared stably arrested in G2. By contrast, with fluridone, an additional ABA decrease was observed resulting from an inhibition of its synthesis inside the bud. New leaf primordia were initiated and many figures of mitosis could be observed, indicating that intense activity of cell division occurred after DNA replication. Therefore, the results indicate that, as long as ABA was synthesized inside the buds, cell cycle was arrested in G2 phase and buds remained dormant. Continued in situ ABA biosynthesis appears, therefore, to be required for the maintenance of bud dormancy. (Author's abstract)
Mots-clés Agrovoc : Rosa, culture in vitro, bourgeon, dormance, levée de dormance, ABA
Mots-clés complémentaires : Rosa hybrida
Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement
Auteurs et affiliations
- Le Bris M.
- Michaux-Ferrière Nicole, CIRAD-AMIS-BIOTROP (FRA)
- Jacob Y.
- Poupet A.
- Barthe P.
- Guigonis J.M.
- Le Page Degivry M.T.
Autres liens de la publication
- Document en bibliothèque
- Localisation du document : CD_BR8277 [(Bibliothèque de Lavalette)]
Source : Cirad - Agritrop (https://agritrop.cirad.fr/391967/)
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