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Inter-Alu-like species-specific sequences in the Saccharum complex

Alix Karine, Paulet Florence, Glaszmann Jean-Christophe, D'Hont Angélique. 1999. Inter-Alu-like species-specific sequences in the Saccharum complex. Theoretical and Applied Genetics, 99 : pp. 962-968.

Journal article ; Article de revue à facteur d'impact
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Abstract : #Alu# sequences constitute the most abundant family of short interspersed nuclear elements, SINEs, in the primate genome. The #Alu#-PCR method, which consists of amplification between "Alu# sequences, is usually applied in human genetics to provide polymorphic markers. Here we report the presence of #Alu#-like sequences in sugarcane and related species by applying the #Alu#-PCR-like method. Amplifications using a PCR primer defined in conserved regions of #Alu# human sequences lead to specific complex multiband profiles in all the #Saccharum# and related genera clones surveyed. The isolation and characterisation of the amplified genus-specific inter-#Alu#-like fragments allowed us to isolate repeated sequences that are specific for different genera of the #Saccharum# complex: MsCIR2 from #Miscanthus#, EaCIR6 and EaCIR7 from #Erianthus#, and SrCIR2 from #Saccharum#. Two PCR diagnostic tests were developed from the inter-#Alu#-like sequences MsCIR2 and EaCIR6, and proved efficient in identifying intergeneric hybrids #Saccharum# x #Miscanthus# or #Saccharum# x #Erianthus#, respectively. The present study illustrates how the #Alu#-PCR-like method could help investigating the origin of amphiploid species and monitoring introgression in plants. (Author's abstract)

Mots-clés Agrovoc : Saccharum, Séquence nucléotidique, PCR, Identification, Empreinte ADN

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

  • Alix Karine
  • Paulet Florence, CIRAD-CA-Canne à sucre (FRA)
  • Glaszmann Jean-Christophe, CIRAD-AMIS-BIOTROP (FRA) ORCID: 0000-0001-9918-875X
  • D'Hont Angélique, CIRAD-CA-Canne à sucre (FRA)

Autres liens de la publication

  • Document en bibliothèque
  • Localisation du document : CD_BR8262 [(Bibliothèque de Lavalette)] ; CD_PE378 [(Bibliothèque de Lavalette)]

Source : Cirad - Agritrop (https://agritrop.cirad.fr/392201/)

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