Kemp S.J., Maillard Jean-Charles, Teale A.J.. 1993. A polymorphism in the bovine gamma-s-crystallin gene revealed by allele-specific amplification. Animal Genetics, 24 (2) : p. 125. ILRAD Laboratoire international pour la recherche sur les maladies animales, Nairobi, Kenya, 27 September 1987/1 October 1987.
Autre titre : Polymorphisme du gène bovin gamma-s-crystalline révélé par amplification spécifique de l'allèle
Abstract : A polymorphism was detected in the 3' untranslated region of the bovine gamma-s-crystallin gene by direct sequencing of polymerase chain reaction (PCR) products nom genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele-specific amplification. The two allele-specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism. (Résumé d'auteur)
Mots-clés Agrovoc : Bovin, PCR, Polymorphisme génétique
Classification Agris : L10 - Animal genetics and breeding
Auteurs et affiliations
- Kemp S.J.
- Maillard Jean-Charles
- Teale A.J.
Autres liens de la publication
- Localisation du document : BA_PEBA899 [(Bibliothèque de Baillarguet)]
Source : Cirad - Agritrop (https://agritrop.cirad.fr/400699/)
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