In vitro cultivation of Phytomonas from latex and phloem-restricte d Phytomonas

Gargani Daniel, Menara C., Segur C., Dollet Michel. 1992. In vitro cultivation of Phytomonas from latex and phloem-restricte d Phytomonas. In : #Phytomonas# workshop = [Second atelier international #Phytomonas#]. s.l. : s.n., 1 p. International Phytomonas Workshop. 2, Santa Marta, Colombie, 5 February 1992/8 February 1992.

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Autre titre : Culture in vitro de Phytomonas à partir de latex et de phloème limité de Phytomonas

Abstract : The #in vitro# culture of #Phytomonas# was the gain obstacle to their study. In and after 1982 we were able to grow #Phytomonas# from laticiferous plants: #Euphorbia pinea#, #E. hirta#, #E. hyssopifolia#, #Mandevilla scabra#, from different areas of the world. But phloem-restricted #Phytomonas# remained difficult to grow and it was not until 1986 we obtained the first primoculture of #Phytomonas# associated with Hartrot disease of coconut, in a medium containing insect cells. After several passages axenic cultures have been obtained. Today we are growing 9 isolates of Hartrot of French Guiana, one from Brazil and two from Venezuela. #Phytomonas# associated with Marchitez disease of oil palm were cultured for the first time in 1989. Four isolates from Colombia are now being cultured. Four of these phloem-restricted isolates have been cloned (37 clones). Recently #Phytomonas# (apparently phloem-restricted) were discovered in Grenada by P. HUNT in #Alpinia purpurata#. Two isolates were obtained in insect cell cultures in the laboratory. (Résumé d'auteur)

Mots-clés Agrovoc : Tissu vasculaire, Phloème, Phytomonas, Culture in vitro, Latex, Euphorbia, Expérimentation en laboratoire

Mots-clés géographiques Agrovoc : France

Classification Agris : H20 - Plant diseases

Auteurs et affiliations

  • Gargani Daniel
  • Menara C.
  • Segur C.
  • Dollet Michel

Autres liens de la publication

Source : Cirad - Agritrop (

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