Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies

Rang Cécile, Bes Martine, Moar William J., Frutos Roger. 1997. Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies. FEBS Letters, 412 : 587-591.

Autre titre : De la nécessité de la production simultanée des protéines 34-kDA et 40-kDa de Bacillus thuringiensis subsp. thompsoni pour la formation des corps d'inclusion

Résumé : Cooperation of two crystal proteins from #Bacillus thuringiensis# subsp. #thompsoni#. strain HnC was shown to be essential for the formation of inclusion bodies. Expression of the operon containing the 34-kDa and 40-kDa protein genes from HnC in a #B. thuringiensis# crystal minus strain resulted in the formation of inclusion bodies identical to those from strain HnC. Interruption of one of the genes in the operon led to the lack of inclusion body and to low production of the remaining protein. Absence of inclusion body and low rate of protein production were also observed when both genes were simultaneously expressed but on different vectors. To show a cooperative effect in the formation of the inclusion body, both proteins must be produced from the same transcript.

Mots-clés Agrovoc : Bacillus thuringiensis, expression des gènes, protéine

Classification Agris : H20 - Maladies des plantes
U30 - Méthodes de recherche

Auteurs et affiliations

• Rang Cécile, CIRAD-CP-CAFE (FRA)
• Bes Martine, CIRAD-GERDAT-BIOTROP/IGEPAM (FRA)
• Moar William J.
• Frutos Roger, CIRAD-GERDAT-BIOTROP/IGEPAM (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/401071/)

Métriques

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