Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe

Urbino Cica, Gérion A., Caruana Marie-Line. 1998. Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe. In : 2nd international workshop on #Bemisia# and geminiviral diseases. Program and abstracts = [Deuxième séminaire international sur les maladies à #Bemisia# et gemini virus. Programme et résumés]. Mayer R.T. (ed.), Maxwell D.P. (ed.). s.l. : s.n., p. 36. International Workshop on Bemisia and Geminiviral Diseases. 2, San Juan, Porto Rico, 7 June 1998/12 June 1998.

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Autre titre : Caractérisation biologique et moléculaire de trois isolats de geminivirus infectant la tomate en Guadeloupe

Abstract : Since 1989, tomato fields in Guadeloupe have been infested by a geminivirus disease. Symptoms are yellow mosaic, chlorotic mottling, leaf rolling and stunting. A survey showed that the disease was present over all the island. Cloning and sequencing of an isolate lead to the identification of a bipartite geminivirus close to potato yellow mosaic virus (PYMV-G) (Polston et al, 1998). In this study, three other virus isolates were collected from tomato in Grande-Terre and on the leeward coast of Basse-Terre and from whiteflies (Bemisia argentifolii) in Grande-Terre. They were characterized biologically (mechanical and vector transmission, host range) and molecularly. PCR primers based on conserved sequences were used to amplify and sequence the intercistronic region and the N-terminal region of the coat protein gene (CP). Sequence comparisons between these isolates and the clone of PYMV-G were established in order to confirm the occurrence of PYMV in Guadeloupe. (Résumé d'auteur)

Mots-clés Agrovoc : Géminivirus, Identification, Solanum

Mots-clés géographiques Agrovoc : Guadeloupe

Classification Agris : H20 - Plant diseases

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