Agritrop
Home

Characterization of 18 new BoLA-DRB3 alleles

Maillard Jean-Charles, Renard C., Chardon P., Chantal Isabelle, Bensaïd Albert. 1999. Characterization of 18 new BoLA-DRB3 alleles. Animal Genetics, 30 : pp. 200-203.

Journal article ; Article de revue à facteur d'impact
Full text not available from this repository.

Autre titre : Caractérisation de 18 nouveaux alléles BoLA-DRB3

Abstract : The second exon of the bovine MHC class II DRB3 gene was amplified by polymerase chain reaction (PCR) from DNA samples of 568 zebu Brahman cattle (Bos indicus) from Martinique (French West Indies). Cloning of these PCR products allowed the isolation of both alleles from each animal, which were characterized by the PCR-restriction fragment length polymorphism (RFLP) technique using the restriction enzymes Rsal, BstYl and Haelll. Four new PCR-RFLP patterns were obtained by digestion with Rsal. These patterns were named 'v', w,'x' and 'y' continuing the accepted nomenclature. Sequencing of each allele allowed the identification of 18 new BoLA-DRB3 exon 2 nucleotide sequences and their deduced amino acid sequences. (Résumé d'auteur)

Mots-clés Agrovoc : Bovin, Zébu, Séquence nucléotidique, Polymorphisme génétique, PCR, RFLP

Mots-clés géographiques Agrovoc : Martinique

Classification Agris : L10 - Animal genetics and breeding

Auteurs et affiliations

  • Maillard Jean-Charles, CIRAD-EMVT-SANTE ANIMALE (FRA)
  • Renard C.
  • Chardon P.
  • Chantal Isabelle, CIRAD-EMVT-SANTE ANIMALE (FRA) ORCID: 0000-0002-3429-484X
  • Bensaïd Albert, CIRAD-EMVT-SANTE ANIMALE (GLP)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/401324/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-02-26 ]