High and low responsiveness of bovine lymphocytes to Trypanosoma brucei in vitro: Lack of correlation with resistance to trypano somiasis

Fumoux F., Traoré Leroux T., Quéval Rémy, Pinder M., Roelants G.E.. 1985. High and low responsiveness of bovine lymphocytes to Trypanosoma brucei in vitro: Lack of correlation with resistance to trypano somiasis. Immunology, 54 : 195-203.

Autre titre : Réponse des lymphocytes bovins à Trypanosoma brucei in vitro : manque de corrélation avec la trypanotolérance

Matériel d'accompagnement : 1 micro-fiche numéro VT851126

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Bovine peripheral blood lymphocytes (PBL) were stimulated to proliferate in vitro by live, irradiated or freeze-thawed Trypanosoma brucei but not by the isolated variant surface glycoprotein. The optimal dose was 10 exponent 5 trypanosomes per 5 X 10 exponent 5 lymphocytes in O.2 ml. Maximal proliferation was at day 5. Of the 98 cattle tested, 36 were high-responders (stimulation indexes 20-104), 49 were low or non-responders (SI 1-10) and 13 were intermediate. The responder status of individual animals did not change over a period of 1 year, nor did it after following deliberate trypanosome infection. The stimulation was dependent on macrophage/monocyte type accessory cells, and this co-operation did not seem to be MHC restricted. Lack of stimulation of non-responder PBL did not appear to be due to the activation of suppressor cells. Accessory cells from non-responder animals could complement PBL from responders, but accessory cells from responders could not complement non-responder PBL. Responsiveness is therefore a characteristic of lymphocytes. Analysis of the surface markers of these lymphocytes or the blast cells generated in culture showed that they were a subpopulation of T cells, possibly TH cells. Analysis of PBL from 98 animals, which had been selected for trypanoresistance or trypanosensitivity under natural tsetse fly challenge, failed to establish a correlation between resistance and level of lymphocyte stimulation by trypanosome in vitro

Mots-clés Agrovoc : bovin, lymphocyte, réponse immunitaire, Trypanosoma brucei, trypanosomose, résistance aux maladies

Mots-clés géographiques Agrovoc : Burkina Faso

Mots-clés complémentaires : Trypanotolérance

Classification Agris : L73 - Maladies des animaux
L72 - Organismes nuisibles des animaux

Auteurs et affiliations

• Fumoux F.
• Traoré Leroux T.
• Quéval Rémy
• Pinder M.
• Roelants G.E.

Source : Cirad - Agritrop (https://agritrop.cirad.fr/446926/)

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