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Manual pollination techniques and verification of incompatibility : Implementation of selection trials and data collection

Lachenaud Philippe. 2000. Manual pollination techniques and verification of incompatibility : Implementation of selection trials and data collection. In : Working procedures for cocoa germplasm evaluation and selection : Proceedings of the CFC/ICCO/IPGRI Project workshop, 1-6 February 1998, Montpellier, France. Eskes Albertus (ed.), Engels Johannes M.M. (ed.), Lass R.A. (ed.). IPGRI, CFC, ICCO. Rome : IPGRI, pp. 98-99. ISBN 92-904343-4-1 CFC/ICCO/IPGRI Project Workshop, Montpellier, France, 1 February 1998/6 February 1998.

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Abstract : Pollination in cocoa must be controlled in order to obtain the expected result. In the past, errors in results from hand pollination were identified by using isozymes and more recently, DNA markers. Even with strict control of hand pollination, a small percentage of unwanted fertilizations can still take place. Possible sources of errors are: - Incomplete isolation of flowers (crawling insects may enter the pollination tubes); - Emasculation of flowers before pollination: this unnecessary manipulation may induce selfing and contamination of the pollination tweezers; - Other manipulations of the flowers can contaminate the pistil with self-pollen; - Successive use of different types of pollen, without cleaning the tweezers with alcohol between manipulations. A description is given of a manual pollination technique, including self and crosspollination, the use of mentor pollen to overcome self-incompatibility, and comparison of this "standard" pollination procedure with locally applied methods. (Résumé d'auteur)

Mots-clés Agrovoc : Theobroma cacao, Pollinisation artificielle, Pollinisation croisée, Incompatibilité pollinique, méthode

Classification Agris : F63 - Plant physiology - Reproduction

Auteurs et affiliations

  • Lachenaud Philippe, CIRAD-CP-CACAO (CIV)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/475698/)

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