The diversity of insect and plant trypanosomatids: molecular (PCR) and morphology data

Podlipaev Sergei A., Frolov A., Bulat S., Banuls A.L., Dollet Michel. 1998. The diversity of insect and plant trypanosomatids: molecular (PCR) and morphology data. In : The flagellates : International Symposium of the Systematics Association, Birmingham, September 7th-10th, 1998. s.l. : s.n., p. 30. Systematics Association International Symposium, Birmingham, Royaume-Uni, 7 September 1998/10 September 1998.

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Additional Information : Résumé seul

Abstract : The most unreliable part in the taxonomic system of the trypanosomatids is the domain of trypanosomatids from insects and plants. The relationships within insect trypanosomatids are still obscure. Twenty-two isolates from insects (both reference strains and recent isolates) and four isolates from plants were, investigated by RAPD, UP-PCR and cross dot-blot hybridization of UP-PCR products. Ultrastructure and some additional characters (polymorphism of colonies on solid medium, DNA content by flow cytometry etc.) were also examined. It was concluded that: I. The main clusters on dendrograms derived from PCR are supported by ultrastructural data; 2. Leptomonas-like isolates are very heterogeneous by all methods used thus confirming the view of Leptomonas as an artificial group; 3. The number of current genera of trypanosomatids is not enough to demonstrate the real diversity of these parasites and new genera have to be established. (Résumé d'auteur)

Mots-clés Agrovoc : Parasite, Taxonomie, Biologie moléculaire, Ultrastructure, Polymorphisme

Mots-clés complémentaires : Trypanosomatidae

Classification Agris : H10 - Pests of plants
F30 - Plant genetics and breeding

Auteurs et affiliations

  • Podlipaev Sergei A., Russian Academy of Science (RUS)
  • Frolov A., Russian Academy of Science (RUS)
  • Bulat S., Institute of Nuclear Physics (RUS)
  • Banuls A.L., ORSTOM [Institut français de recherche scientifique pour le développement en coopération] (FRA)

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