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A method to estimate acetylcholinesterase-active sites and turnover in insects

Charpentier Alice, Menozzi Philippe, Marcel Véronique, Villate François, Fournier Didier. 2000. A method to estimate acetylcholinesterase-active sites and turnover in insects. Analytical Biochemistry, 285 (1) : pp. 76-81.

Journal article ; Article de revue à facteur d'impact
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Abstract : Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy) -1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific. (Résumé d'auteur)

Mots-clés Agrovoc : Insecticide, Acétylcholinestérase, Propriété des pesticides, Résistance aux pesticides, Lutte anti-insecte, Lutte chimique, Drosophila melanogaster, Activité enzymatique, Inhibition

Classification Agris : H10 - Pests of plants

Auteurs et affiliations

  • Charpentier Alice, Université Toulouse III Paul Sabatier (FRA)
  • Menozzi Philippe, CIRAD-CA-COTON (FRA)
  • Marcel Véronique, Université Toulouse III Paul Sabatier (FRA)
  • Villate François, Université Toulouse III Paul Sabatier (FRA)
  • Fournier Didier, Université Toulouse III Paul Sabatier (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/476765/)

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