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A highly sensitive, non radioactive assay for T cell activation in cattle : applications in scereening for antigens recognised by CD4+ and CD8+ T cells

Ballingall Keith T., Mwangi Duncan M., MacHugh Niall D., Taracha Evans L.N., Totté Philippe, McKeever Declan J.. 2000. A highly sensitive, non radioactive assay for T cell activation in cattle : applications in scereening for antigens recognised by CD4+ and CD8+ T cells. Journal of Immunological Methods, 239 : pp. 85-93.

Journal article ; Article de revue à facteur d'impact
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Abstract : We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNy and activation of immune CD4 + and CD8 + T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4 + T cell clones. (Résumé d'auteur)

Mots-clés Agrovoc : Cellule, Immunité cellulaire, Bovin, Antigène, Immunologie

Mots-clés complémentaires : Cellule endothéliale bovine

Classification Agris : L50 - Animal physiology and biochemistry

Auteurs et affiliations

  • Ballingall Keith T., ILRI (KEN)
  • Mwangi Duncan M., ILRI (KEN)
  • MacHugh Niall D., ILRI (KEN)
  • Taracha Evans L.N., ILRI (KEN)
  • Totté Philippe, CIRAD-EMVT-SANTE ANIMALE (GLP)
  • McKeever Declan J., ILRI (KEN)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/478228/)

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