Mapping candidate genes in Eucalyptus with emphasis on lignification genes

Gion Jean-Marc, Rech Philippe, Grima Pettenati Jacqueline, Verhaegen Daniel, Plomion Christophe. 2000. Mapping candidate genes in Eucalyptus with emphasis on lignification genes. In : Conférence sur les Nouveaux horizons en sciences et biotechnologies végétales, 5-9 mars 2000, Toulouse-Labège (France) : programme, résumés = Conference on New frontiers in plant science and plant biotechnology, 5-9 March 2000, Toulouse-Labège (France) : programme, abstracts. IFR FR40. Castanet Tolosan : Institut fédératif de recherche FR40, Résumé, p. 29. Colloque sur les Nouveaux Horizons en Sciences et Biotechnologies Végétales. 1, Toulouse, France, 5 March 2000/9 March 2000.

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Abstract : We used the single-strand conformation polymorphism (SSCP) technique to map eight genes on Eucalyptus urophylla and Eucalyptus grandis linkage maps. These included four genes involved in the common phenylpropanoid pathway (Caffeic acid 3-0-methyltransferase, Caffeoyl CoA 3-0-methyltransferase, 4-Coumarate CoA ligase and phenylalanine ammonia lyase), two genes involved in the "lignin specific" pathway (Cinnamoyl CoA reductase and Cinnamyl alcohol dehydrogenase), and two symbiosis regulated genes (EgHypar and EgTubAl). A novel source of variation which affects the SSCP pattern, i.e. presence or absence of electrophoresis buffer upon loading the samples into the polyacrylamide gel, was found. The placement of these genes on the Eucalyptus maps was carried out using an interspecific hybrid mapping population. This will further facilitate the identification or exclusion of "positional" candidate genes for characterizing quantitative trait loci (QTL) for wood quality and vegetative propagation related traits. (Texte intégral)

Mots-clés Agrovoc : Eucalyptus urophylla, Eucalyptus grandis, Gène, Lignification, Carte génétique

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

  • Grima Pettenati Jacqueline, CNRS (FRA)
  • Plomion Christophe, INRA (FRA)

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