Functional expression of the catalytic domains of two cysteine proteinases from Trypanosoma congolense

Boulangé Alain, Serveau Carole, Brillard M., Minet Cécile, Gauthier Francis, Diallo Adama, Lalmanach Gilles, Authié Edith. 2001. Functional expression of the catalytic domains of two cysteine proteinases from Trypanosoma congolense. International Journal for Parasitology, 31 : 1435-1440.

Résumé : The catalytic domains of two closely related cysteine proteinases (CP1 and CP2) from Trypanosoma congolense, referred to as C1 and C2, were expressed as proforms in Escherichia coli (C1) and in the baculovirus system (C1 and C2). While the bacterial expression system did not allow recovery of active C1, the baculovirus system led to secretion of inactive zymogens which could be processed at acidic pH into mature enzymes. Active C1 and C2 were purified from serum-free culture supernatants by anion-exchange chromatography and characterised. Their kinetic parameters and pH activity profiles confirmed the relatedness between C2 and native CP2 (congopain). These properties also underline major functional differences between C1 and C2, that appear to relate to discrete but essential sequence differences. It is likely that these two enzymes perform distinct roles in vivo, in the parasite and/or in the host-parasite relationships.

Classification Agris : L72 - Organismes nuisibles des animaux

Auteurs et affiliations

• Boulangé Alain, CIRAD-EMVT-SANTE ANIMALE (KEN) ORCID: 0000-0002-8505-7465
• Serveau Carole, Université François Rabelais (FRA)
• Brillard M., Université François Rabelais (FRA)
• Minet Cécile, CIRAD-EMVT-SANTE ANIMALE (FRA) ORCID: 0000-0002-9287-1481
• Gauthier Francis, Université François Rabelais (FRA)
• Diallo Adama, CIRAD-EMVT-SANTE ANIMALE (AUT)
• Lalmanach Gilles, Université François Rabelais (FRA)
• Authié Edith, CIRAD-EMVT-SANTE ANIMALE (KEN)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/483322/)

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