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Exchange of domain I from Bacillus thuringiensis Cry 1 toxins influences protoxin stability and crystal formation

Rang Cécile, Vachon Vincent, Coux Florence, Carret Céline, Moar William J., Brousseau Roland, Schwartz Jean Louis, Laprade Raynald, Frutos Roger. 2001. Exchange of domain I from Bacillus thuringiensis Cry 1 toxins influences protoxin stability and crystal formation. Current Microbiology, 43 : 1-6.

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Résumé : Influence of domain I exchange on the stability and production of Bacillus thuringiensis Cry1 protoxins as well as on the shape of inclusion and toxicity to Spodoptera exigua and Plutella xylostella larvae was investigated. Chimeric genes were prepared by exchanging the regions coding for domain I between Cry1Aa, Cry1Ab, Cry1Ac, Cry1C, and Cry1E. The AcCC chimera accumulated into bipyramidal inclusion bodies, whereas CEE produced round-shaped inclusion bodies, and ECC and AaEE protoxins produced small granules. AbEE and EAaAa did not produce any inclusion body and were visualized by immunodetection only. AcCC, CEE, ECC, and AaEE were stable to trypsin, whereas AbEE and EAaAa were not. Bioassays showed that the chimeras were not toxic in vivo. However, S. exigua larvae fed with the activated AcCC toxin displayed a lower growth rate.

Mots-clés Agrovoc : Bacillus thuringiensis, biopesticide, lutte anti-insecte, toxine bactérienne, transfert de gène, Spodoptera exigua, Plutella xylostella

Classification Agris : H10 - Ravageurs des plantes

Auteurs et affiliations

  • Rang Cécile, CIRAD-AMIS-BIOTROP (FRA)
  • Vachon Vincent, Université de Montréal (CAN)
  • Coux Florence, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
  • Carret Céline
  • Moar William J., Auburn University (USA)
  • Brousseau Roland, Biotechnology Research Institute (CAN)
  • Schwartz Jean Louis, Université de Montréal (CAN)
  • Laprade Raynald, Université de Montréal (CAN)
  • Frutos Roger, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/488233/)

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