Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.)

Assani A., Haïcour R., Wenzel Gerhard, Foroughi-Wehr Barbel, Bakry Frédéric, Côte François-Xavier, Ducreux Georges, Ambroise Annick, Grapin Agnès. 2002. Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.). Plant Science, 162 (3) : pp. 355-362.

Journal article ; Article de revue à facteur d'impact
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Abstract : Among the various strategies for genetic improvement in banana, somatic hybridisation appears to be a promising complement to classical breeding since protoplasts are amenable to plant regeneration. Therefore, the present investigation was undertaken to improve protoplast regeneration in banana. Protoplasts were isolated from young leaves, calli and embryogenic cell suspensions. The highest protoplast yield was obtained with cell suspensions. Mesophyll protoplasts and callus-derived protoplasts did not divide. However, protoplasts isolated from cell suspension developed into plants. Feeder cells and protoplast culture at high density were required for plant regeneration from protoplasts. Plant regeneration through somatic embryogenesis was achieved in 'Grande Naine' and 'Gros Michel' (#Musa# spp. Sub-Group 'Cavendish' AAA), 'Currare Enano' and 'Dominico' (Sub-Group plantain AAB), SF265, IRFA903 and Col49 (Sub-Group AA). The frequency of embryo formation and plant regeneration was genotype-dependent. Large number of somatic embryos and regenerated plants were produced in SF265 (AA) followed by 'Domenico' (AAB) and 'Grande Naine' (AAA). (Résumé d'auteur)

Mots-clés Agrovoc : Musa, Régénération in vitro, Protoplaste, Technique de culture, Embryogénèse somatique, Génotype, Cellule, Amélioration des plantes, Embryon somatique, Culture de cellule

Mots-clés complémentaires : Suspension, Régénération de plante

Classification Agris : F30 - Plant genetics and breeding
F02 - Plant propagation

Auteurs et affiliations

  • Assani A., Université de Paris-Sud (FRA)
  • Haïcour R., Université de Paris-Sud (FRA)
  • Wenzel Gerhard, Technische Universität München (DEU)
  • Foroughi-Wehr Barbel, BAZ (DEU)
  • Bakry Frédéric, CIRAD-FLHOR-BPA (FRA)
  • Côte François-Xavier, CIRAD-FLHOR-BPA (FRA)
  • Ducreux Georges, Université de Paris-Sud (FRA)
  • Ambroise Annick, Université de Paris-Sud (FRA)
  • Grapin Agnès

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