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Chain-length selectivity of various lipases during hydrolysis, esterification and alcoholysis in biphasic aqueous medium

Vaysse Laurent, Ly Aboubakry, Moulin Guy, Dubreucq Eric. 2002. Chain-length selectivity of various lipases during hydrolysis, esterification and alcoholysis in biphasic aqueous medium. Enzyme and Microbial Technology, 31 (5) : pp. 648-655.

Journal article ; Article de revue à facteur d'impact
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Abstract : Chain-length selectivity profiles of seven lipases (EC 3.1.1.3) have been determined by multiple substrate competition for three reactions at 30 and 50°C: hydrolysis and synthesis of fatty acid methyl esters and acyl transfer between fatty acid ethyl esters and methyl esters. All acyl chains were even-numbered and saturated. Chain-length specificity profiles depended on the enzyme and the reaction considered, due to the combined influence of enzyme and substrate properties. Changing temperature had a significant influence on substrate specificity for esterification only. The results led to the selection of Candida deformans lipase for the selective hydrolysis of short-chain (C8, C10) esters in an equimolar mixture of C8-C18 methyl esters in the presence of various methanol concentrations. The reaction resulted in a long-chain-enriched methyl ester and a short-chain-enriched fatty acid fractions, respectively, consisting of 96% C12-C18 and 68% C8-C10 in a given example. (Résumé d'auteur)

Mots-clés Agrovoc : Triacylglycérol lipase, Hydrolyse, Estérification, Bioconversion, Activité enzymatique, Acide gras

Mots-clés complémentaires : Substrat, Candida deformans, Transestérification, Sélectivité

Classification Agris : Q60 - Processing of non-food or non-feed agricultural products

Auteurs et affiliations

  • Vaysse Laurent, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
  • Ly Aboubakry, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
  • Moulin Guy, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
  • Dubreucq Eric, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/509619/)

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