Endogenous Banana streak virus sequences in Musa genome : a new risk for breeding program

Lheureux Fabrice, Carreel Françoise, Jenny Christophe, Laboureau Nathalie, Lockhart Benham E.L., Iskra Caruana Marie-Line. 2002. Endogenous Banana streak virus sequences in Musa genome : a new risk for breeding program. In : The world of microbes : XIIth International Congress of Virology, Paris, 27th July to 1st August 2002. SFM, IUMS. Paris : EDK, Résumé, p. 227. International Congress of Virology. 11, Paris, France, 27 July 2002/1 August 2002.

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Abstract : Inter-specific #Musa# hybrids have been found to be infected with Banana streak pararetrovirus Obino I'Ewai strain (BSV-Ol) responsible of Banana Streak Disease (BSD). One hypothesis suggest (i) that BSD occurrence in these hybrids results from activation of BSV-Ol endogenous pararetrovirus sequences (EPRVs) integrated into the #Musa# genome rather than from external sources of infection and (ii) that the process of genetic hybridisation may be one factor involved in triggering episomal expression of the BSV integrants. In order to test this hypothesis we carried out a genetic analysis of BSD incidence in a F1 triploid (AAB) population produced by hybridisation between virus- and disease-free #M. balbisiana# (BB) and #M. acuminata# (AAAA) parents. Half of the F1 progeny expressed BSV particles. Ten AFLP markers co-segregating with the absence and/or presence of BSV infection were identified in the #M. balbisiana# genome only. Analysis of the segregation of these markers allowed the construction of a genetic map containing the focus associated with BSV infection called BSV Express Locus (BEL). A genetic mechanism is involved in BSV appearance, conferring the role of carrier to the #M. balbisiana# parent. Two previously unidentified BSV strains in addition to BSV-Ol, have been furthermore detected in these diseased hybrids: BSV-Imové (BSV-Im) and BSV-Gold Finger (BSV-GF) strains. The BSV-Ol and BSV-Im strains appeared in almost all diseased hybrids while the BSV-GF strain appeared only in about half of these hybrids. The close relationships between the presence of these two new BSV strains and the presence of corresponding EPRVs in the #M. balbisiana# parent genome shows that the #M. balbisiana# genome contains at least two other pathogenic BSV EPRVs: BSV-Im and BSV-GF EPRVs. Genetic analysis resulting from AFLP results showed that BSV-0l and BSV-Im EPRVs' expression depends on the same genetic factor, the BEL locus. Although the BSV-GF is not genetically linked to the BEL locus, the BSV-GF strain appearance and the activation of corresponding EPRV(s) seem to be also subordinated to the BEL locus. So, the discovery of new BSV EPRVs linked to BEL locus implicates that BEL differs in nature from BSV EPRVs. Our interest is now the understanding of (i) the mechanism of activation of these strains resulting in BSV expression from EPRVs and (ii) the apparent resistance of the #M. balbisiana# parent against BSV expression and external infection. (Résumé d'auteur)

Mots-clés Agrovoc : Musa, Virus des végétaux, Génome, Séquence nucléotidique, Amélioration des plantes, Retroviridae, Carte génétique, mécanisme de défense, Résistance aux maladies

Mots-clés complémentaires : Mosaïque en tirets, AFLP

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Auteurs et affiliations

  • Lheureux Fabrice, CIRAD-FLHOR-BPA (FRA)
  • Carreel Françoise, CIRAD-FLHOR-BPA (GLP)
  • Jenny Christophe, CIRAD-FLHOR-BPA (GLP)
  • Laboureau Nathalie, CIRAD-FLHOR-BPA (FRA)
  • Lockhart Benham E.L., University of Minnesota (USA)
  • Iskra Caruana Marie-Line, CIRAD-AMIS-PROTECTION DES CULTURES (FRA) ORCID: 0000-0003-4486-2449

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