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Molecular identification of Diadegma species (Ichneumonidae) parasitising diamondback moth Plutella xylostella (Plutellidae) in Eastern and Southern Africa

Wagener B., Löhr Bernhard, Reineke A., Zebitz C.P.W.. 2004. Molecular identification of Diadegma species (Ichneumonidae) parasitising diamondback moth Plutella xylostella (Plutellidae) in Eastern and Southern Africa. In : Improving biocontrol of Plutella xylostella : Proceedings of the International Symposium, Montpellier, France, 21-24 October 2002. Kirk Alan A. (ed.), Bordat Dominique (ed.). CIRAD, USDA-ARS. Montpellier : CIRAD, 252-256. ISBN 2-87614-570-7 International Symposium Improving Biocontrol of Plutella xylostella, Montpellier, France, 21 Octobre 2002/24 Octobre 2002.

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Résumé : Sequencing of the second internal transcribed spacer (ITS2) of the ribosomal DNA followed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis were used to differentiate Diadegma species (Ichneumonidae) parasitising Diamond back moth DBM in eastern and southern Africa. The maximum nucleotide divergence between D. mollipla and D. semiclausum was 6.8%, whereas D. sp. from Ethiopia differs in - 35%, The intraspecific divergence was 0.3% in Diadegma mollipla, and 0.7% in Diadegma semiclausum.

Mots-clés Agrovoc : Diadegma, identification, séquence nucléotidique, parasitoïde, Plutella xylostella

Mots-clés géographiques Agrovoc : Afrique

Classification Agris : H10 - Ravageurs des plantes

Auteurs et affiliations

  • Wagener B., ICIPE (KEN)
  • Löhr Bernhard, ICIPE (KEN)
  • Reineke A., Institute of Phytomedicine (DEU)
  • Zebitz C.P.W., Institute of Phytomedicine (DEU)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/512012/)

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