Interleukin-2 expression in PBMC from sheep with different IL-2 genotypes

Lühken Gesine, Stamm Ivonne, Menge Christian, Erhardt G.. 2002. Interleukin-2 expression in PBMC from sheep with different IL-2 genotypes. In : Second international symposium on Candidate Genes for Animal Health (C.G.A.H), Montpellier, France, August 16-18th 2002 : abstracts. CIRAD, INRA. Montpellier : CIRAD, Résumé, 1 p. International Symposium on Candidate Genes for Animal Health. 2, Montpellier, France, 16 August 2002/18 August 2002.

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Additional Information : Session 2 : Mechanisms of defence against infections

Abstract : The transcription factor GATA-3 appears to be a regulator of Th1/Th2 digerentiation, which is able to influence resistance or susceptibility to infectious diseases. From murine interleukin-4 it is known that a DNA-binding site for the GATA-family of transcription factors in the second intron of the gene has enhancer activity. Furthermore, sequence alteration of the binding site leads to a reduced enhancer activity. We identified a putative GATA binding site in the second intron of the ovine interleukin-2 gene (IL-2), which also has been conserved in the second intron of the interleulkin-2 genes of other species like cattle, goat, pig, chicken and man. In the sheep breeds Romanov and German Grey Heath, we found within this sequence a nucleotide transition (G->C) at position +446 of the gene. We compared the expression levels of IL-2 in peripheral blood mononuclear cells (PBMC) isolated from adult and healthy female Romanov sheep (n = 12) of different genotypes (CC, GG, GC) regarding the potential binding motif. The IL-2 mRNA expression in PBMC without and after stimulation for 4h with concanavalin A (ConA) or phorbol-12-myristate-13-acetate in combination with ionomycin (PMA/I) has been characterized by real-time reverse transcriptase polymerase chain reaction (RT-PCR). After stimulation. the IL-2 mRNA expression increased in average 80-fold and 30-fold, with ConA and PMA/1, respectively. Our results showed no significant differences in IL-2 mRNA expression between the three groups of genotypes. This would be maintained by our first results in electromobility shift assays with nuclear extract including GATA-3, leading to a gel retardation both with the "normal" sequence and with the mutated motif. (Texte intégral)

Mots-clés Agrovoc : Expression des gènes, Interleukine, Mouton

Classification Agris : L10 - Animal genetics and breeding

Auteurs et affiliations

  • Lühken Gesine, Justus Liebig University (DEU)
  • Stamm Ivonne, Institute for Hygiene and Infectious Diseases of Animals (DEU)
  • Menge Christian, Institute for Hygiene and Infectious Diseases of Animals (DEU)
  • Erhardt G., Justus Liebig University (DEU)

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