Detecting QTL controlling genetic susceptibility to Salmonella abortus ovis vaccinal strain RV6 in INRA401 sheep

Moreno C.R., Elsen Jean-Michel, François D., Pitel F., Bouix J., Brunel J.C., Weisbecker J.L., Schibler L., Goudarzi K., Cribiu E.P., Lantier I., Berthon P., Gautier A., Boivin R., Lantier F.. 2002. Detecting QTL controlling genetic susceptibility to Salmonella abortus ovis vaccinal strain RV6 in INRA401 sheep. In : Second international symposium on Candidate Genes for Animal Health (C.G.A.H), Montpellier, France, August 16-18th 2002 : abstracts. CIRAD, INRA. Montpellier : CIRAD, Résumé, 1 p. International Symposium on Candidate Genes for Animal Health. 2, Montpellier, France, 16 August 2002/18 August 2002.

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Additional Information : Session 3 : Genetic resistance / susceptibility to infectious diseases (bacteria / virus)

Abstract : In Human and animals, salmonellosis are responsible of several pathologies: pneumonia, abortion, enteritis, septicaemia. In mice, the salmonellosis resistance mechanisms have been shown to be influenced by several genes, including the NRAMP1 (Natural resistance-associated macrophage protein 1) gene which has a major effect. Other studies showed that the Nramp mice gene is conserved in bird and mammalian species including sheep where it has been located on sheep chromosome 2. An experimental population (1216 lambs from 30 sires) of INRA401 sheep was created to test for the effect on salmonellosis resistance of the candidate Nramp1 gene and to search for other QTLs localised on the whole genome controlling this resistance. INRA401 is a sheep composite line developed from crosses between Berrichon du Cher and Romanov breeds. At 113 days of age on average, lambs were inoculated intravenously with 108 Salmonella abortus ovis RV6 (vaccinal strain). They were slaughtered 10 days after the inoculation. To estimate the salmonellosis genetic resistance of lambs. several traits were measured at inoculation and/or slaughtering: IgM and IgG1 antibody rates, body weight, spleen and pre-scapular nodes weights and number of Salmonella persisting in these organs. Traits were analysed with two approaches: quantitative analysis of genetic variability and QTL analysis to search an effect of the candidate NRAMP1 gene or of other genes using genotyping for 100 microsatellite markers. Heritabilities of traits varied between 0.10 and 0.64 (significantly different from zero). Then in sheep like in other species, susceptibility to salmonellosis is genetically controlled. Moreover, correlations between traits are consistent with known immunological mechanisms. The genetic variability observed should help QTL detection. Preliminary unimarker analysis of 30 markers showed that a marker located close to NRAMP1 gene, affects the IgM rate variation between inoculation and slaughtering, and that a marker located close to the MHC genetic complex affects the IgG1 rate variation between inoculation and slaughter. The genotyping of 70 additional markers is in process, and will allow the search of QTLs on the whole genome using interval mapping methods. (Texte intégral)

Mots-clés Agrovoc : Salmonella, Résistance aux maladies, Vaccin

Mots-clés complémentaires : Salmonella abortus

Classification Agris : L10 - Animal genetics and breeding

Auteurs et affiliations

  • Moreno C.R., INRA (FRA)
  • Elsen Jean-Michel, INRA (FRA)
  • François D., INRA (FRA)
  • Pitel F., INRA (FRA)
  • Bouix J., INRA (FRA)
  • Brunel J.C., INRA (FRA)
  • Weisbecker J.L., INRA (FRA)
  • Schibler L., INRA (FRA)
  • Goudarzi K., INRA (FRA)
  • Cribiu E.P., INRA (FRA)
  • Lantier I., INRA (FRA)
  • Berthon P., INRA (FRA)
  • Gautier A., INRA (FRA)
  • Boivin R., INRA (FRA)
  • Lantier F., INRA (FRA)

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