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A targeted comparative map of the region surrounding the bovine spinal dysmyelination locus

Nissen Peter H., Thomsen Bo, Bendixen Christian. 2002. A targeted comparative map of the region surrounding the bovine spinal dysmyelination locus. In : Second international symposium on Candidate Genes for Animal Health (C.G.A.H), Montpellier, France, August 16-18th 2002 : abstracts. CIRAD, INRA. Montpellier : CIRAD, Résumé, 1 p. International Symposium on Candidate Genes for Animal Health. 2, Montpellier, France, 16 August 2002/18 August 2002.

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Additional Information : Session 4 : Genetic resistance / susceptibility to non-infectious diseases

Abstract : Bovine spinal dysmyelination (BSD) is a neurological disease in cattle characterised by dysmyelination in the spinal cord and paralysis in the hind limbs. In order to construct a detailed comparative map of the region on bovine chromosome 11 (BTA 11) that contain the disease gene, the corresponding region in the human genome was targeted by sequencing bacterial artificial chromosomes (BACs), isolated with microsatellite markers flanking the BSD gene and similarity searches against the draft human genome sequence. To verify the results a radiation hybrid (RH) map was constructed, using four microsatellite markers from the BSD region, spanning approximately 9 cM and genes pricked from the human genome based on the BLAST hits using the BAC sequences. Furthermore, a number of genes from human chromosome 2 (HSA2) were mapped based on known or inferred biological function. The produced RH map compared to the human genome map defines three new blocks of synteny conserved between bovine and hum^ and indicates that an evolutionary breakpoint is located between the two markers showing strongest linkage to the BSD gene. Thus, genes from human chromosome 2 q11, p13 and p22 were mapped to a limited region on BTA11. These data will direct further studies towards the identification of the BSD gene. The study further indicates that a strategy, that combines partial sequencing of genomic clones, sequence similarity searches against the human genome sequence, and radiation hybrid mapping of putative orthologs, is a powerful method to target a comparative map to a specific region of interest. (Texte intégral)

Mots-clés Agrovoc : Trouble du système nerveux, Carte génétique

Classification Agris : L10 - Animal genetics and breeding

Auteurs et affiliations

  • Nissen Peter H., Danish Institute of Agricultural Sciences (DNK)
  • Thomsen Bo, Danish Institute of Agricultural Sciences (DNK)
  • Bendixen Christian, Danish Institute of Agricultural Sciences (DNK)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/512105/)

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