Second Gérard, Kahn François, Barcelos Edson.
1998. AFLP analysis is well suited to a quick characterization of the molecular diversity of whole genera. Examples from palms (Elaeis, Astrocaryum) and cassava (Manihot).
Résumé : We applied AFLP to analyze the extent and structure of the molecular diversity among the following genera: - Elaeis (Oil palm). The domesticated species, with major present economic importance, originated in Africa but all the wild relatives are from tropical America. AFLP and RFLP analysis were compared. - Astrocaryum. A major palm genera in the Amazon bassin. Three species are emerging with potential economic value. Morphological systematic is well advanced but the limits of species are controversial. Most species represent good ecological markers. Distributed in most of the neotropical area. - Manihot (Cassava). A most difficult genus in systematic. Many species are found in most of the neotropics. A major challenge in the utilization and the conservation of the genetic resources of a major staple crop. Air dried leaves (heat or silica gel) or small pieces of regular herbarium specimens were generally used to simplify the collection of representative material from most of tropical South America (Astrocaryum, Manihot). Existing collections were sampled for Elaeis. AFLP analysis were contracted to the commercial laboratory Linkage Genetics (Salt Lake City, Utah). Multivariate analysis was used to evidence species relationships and boundaries. Contrasted examples of the results will be presented. While established systematic based on morphology is generally confirmed, new insights in the genera structure were also obtained and hidden structures evidenced. The genus Manihot shows a huge diversity but species boundaries are most difficult to define. The "window" given by AFLP on molecular polymorphism is well adapted to the study of infra-specific and infra-generic diversity. (Texte intégral)
Mots-clés Agrovoc : Elaeis, Astrocaryum, Manihot, variation génétique, RFLP, collection de matériel génétique
Mots-clés complémentaires : AFLP
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Second Gérard, ORSTOM [Institut français de recherche scientifique et technique pour le développement en coopération] (GUF)
- Kahn François, ORSTOM [Institut français de recherche scientifique et technique pour le développement en coopération] (ECU)
- Barcelos Edson, CIRAD-CP-Progamme palmier (BRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/512398/)
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