Agritrop
Accueil

Genotyping field strains of African swine fever virus by partial p72 gene characterisation

Bastos Armanda, Penrith Mary-Louise, Crucière Catherine, Edrich J.L., Hutchings Geoff, Roger François, Couacy-Hymann Emmanuel, Thomson G.R.. 2003. Genotyping field strains of African swine fever virus by partial p72 gene characterisation. Archives of Virology, 148 (4) : 693-706.

Article de revue ; Article de revue à facteur d'impact
[img] Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
512897.pdf

Télécharger (179kB) | Demander une copie

Résumé : A PCR-based sequencing method was developed which permits detection and characterization of African swine fever virus (ASFV) variants within 5 and 48 h, respectively, of receipt of a clinical specimen. Amplification of a 478 bp fragment corresponding to the C-terminal end of the p72 gene, confirms virus presence with genetic characterization being achieved by nucleotide sequence determination and phylogenetic analysis. The method was applied to 55 viruses including those representative of the major ASF lineages identified previously by restriction fragment length polymorphism (RFLP) analysis. Results confirmed that the p72 genotyping method identifies the same major viral groupings. Characterization of additional viruses of diverse geographical, species and temporal origin using the PCR-based method indicated the presence of ten major ASF genotypes on the African continent, the largest of which comprised a group of genetically homogeneous viruses recovered from outbreaks in Europe, South America, the Caribbean and West Africa (the ESAC-WA genotype). In contrast, viruses from southern and East African countries were heterogeneous, with multiple genotypes being present within individual countries. This study provides a rapid and accurate means of determining the genotype of field and outbreak strains of ASF and is therefore useful for molecular epidemiological clarification of ASF.

Mots-clés Agrovoc : maladie des animaux, PCR, analyse biologique, porcin, diagnostic

Classification Agris : L73 - Maladies des animaux

Auteurs et affiliations

  • Bastos Armanda, University of Pretoria (ZAF)
  • Penrith Mary-Louise, ARC (ZAF)
  • Crucière Catherine, AFSSA (FRA)
  • Edrich J.L., University of Pretoria (ZAF)
  • Hutchings Geoff, Institute for Animal Health (GBR)
  • Roger François, CIRAD-EMVT-SANTE ANIMALE (GBR) ORCID: 0000-0002-1573-6833
  • Couacy-Hymann Emmanuel, LANADA (CIV)
  • Thomson G.R., ARC (ZAF)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/512897/)

Voir la notice (accès réservé à Agritrop) Voir la notice (accès réservé à Agritrop)

[ Page générée et mise en cache le 2024-03-26 ]