Assani A., Bakry Frédéric, Kerbellec Florence, Haïcour R., Wenzel Gerhard, Foroughi-Wehr Barbel. 2003. Production of haploids from anther culture of banana (Musa balbisiana (BB)). Plant Cell Reports, 21 (6) : pp. 511-516.
Abstract : We report here, for the first time, the production of haploid plants of banana #Musa balbisiana# (BB). Callus was induced from anthers in which the majority of the microspores were at the uninucleate stage. The frequency of callus induction was 77%. Callus proliferation usually preceded embryo formation. About 8% of the anthers developed androgenic embryos. Of the 147 plantlets obtained, 41 were haploids (n=x=11). The frequency of haploid production depended on genotypes used: 18 haploid plants were produced from genotype 'Pisang klutuk', 12 from 'Pisang batu', seven from 'Pisang klutuk wulung' and four from 'Tani'. The frequency of regeneration was 1.1%, which was based on the total number of anthers cultured. Diploid plants (2n=2x=22) were also observed in the regenerated plants. The haploid banana plants that were developed will be important material for the improvement of banana through breeding programmes. (Résumé d'auteur)
Mots-clés Agrovoc : Musa balbisiana, Haploïdie, Culture d'anthère, Androgénèse, Régénération in vitro, Nombre chromosomique, Amélioration des plantes
Mots-clés complémentaires : Régénération de plante
Classification Agris : F02 - Plant propagation
F30 - Plant genetics and breeding
Auteurs et affiliations
- Assani A., ENSBANA (FRA)
- Bakry Frédéric, CIRAD-FLHOR-BPA (FRA)
- Kerbellec Florence
- Haïcour R., Université de Paris-Sud (FRA)
- Wenzel Gerhard, Technische Universität München (DEU)
- Foroughi-Wehr Barbel, BAZ (DEU)
Autres liens de la publication
- Document en bibliothèque
- Localisation du document : CD_BR11471 [(Bibliothèque de Lavalette)] ; CD_BR11470 [(Bibliothèque de Lavalette)]
Source : Cirad - Agritrop (https://agritrop.cirad.fr/513229/)
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