Lavanya M., Uraichuen Sopon, Pieretti Isabelle, Vassal Jean-Michel, Sharma Kiran, Royer Monique.
2003. Genetic transformation of pigeonpea to evaluate insecticidal genes for resistance to the legume pod borer Helicoverpa armigera [SS1-12].
In : 7th International Congress of Plant Molecular Biology, ISPMB 2003, Barcelona (Spain), June 23-28. Book of abstracts. ISPMB
Résumé : Pigeonpea (Cajanus cajan (L.) Millsp.) is one of the major grain legume (pulse) crop of the tropics and sub tropics and is grown in about 50 countries of Asia, Eastern and Southern Africa and Caribbean for various uses such as food, fodder, firewood and building material. Pigeonpea seed protein on an average approximately 21 percent compares well with that of the other important grain legumes. However, its production and productivity are constrained by several biotic stresses including Helicoverpa armigera or the legume pod borer which is the major and the most economically important pest on pigeonpea. Crop loses due to H. armigera have been estimated to over US dollars 1 billion annually. Intensification of agriculture has exacerbated the pest problem, and farmers are responding by using more toxic pesticides more frequently. Moreover, the levels of resistance to this insect pest in the available pigeonpea germplasm are moderate to low. Therefore, the development of cultivars with resistance to this insect through the expression of insecticidal genes can form an important aspect of integrated pest management. In this study, the insecticidal activity and receptor binding properties of the B. thuringiensis toxins Cry1Aa, Cry1Ab, Cry1Ac and Cry2Aa to H. armigera (BK77) were studied. Molecular constructs to express these four toxins in pigeonpea were prepared for the above genes. Efficient systems for the tissue culture regeneration of pigeonpea have been developed by using the axillary tissue and leaflet explants. The binary vectors developed are being used to transform pigeonpea by using Agrobacterium-mediated genetic transformation for the effective deployment of the transgenic pigeonpea for integrated pest management. (Texte intégral)
Mots-clés Agrovoc : Cajanus cajan, plante transgénique, gène, insecticide, résistance aux organismes nuisibles, Helicoverpa armigera
Classification Agris : H10 - Ravageurs des plantes
F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Lavanya M., ICRISAT (IND)
- Uraichuen Sopon
- Pieretti Isabelle, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
- Vassal Jean-Michel, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
- Sharma Kiran, ICRISAT (IND)
- Royer Monique, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/514094/)
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