Association studies using random and candidate microsatellite loci in two infectious goat diseases

Obexer-Ruff Gabriela, Sattler Ursula, Martinez Dominique, Maillard Jean-Charles, Chartier Christophe, Saitbekova Nasikat, Glowatzki Marie-Louise, Gaillard Claude. 2003. Association studies using random and candidate microsatellite loci in two infectious goat diseases. Genetics Selection Evolution, 35suppl.1, S113-S119 International Symposium on Candidate Genes for Animal Health. 2, Montpellier, France, 16 August 2002/18 August 2002.

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Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : We established a set of 30 microsatellites of Bovidae origin for use in a biodiversity study in Swiss and Creole goats. Additional microsatellites located within or next to "candidate" genes of interest, such as cytokine genes (IL4, INF-gamma) and MHC class II genes (DRB, DYA) were tested in the caprine species in order to detect possible associations with two infectious caprine diseases. Microsatellite analysis was undertaken using automated sequencers (ABI373 & 3100). In the first study, a total of 82 unrelated Creole goats, 37 resistant and 45 susceptible to Heartwater disease (Cowdriosis) were analysed. In this study, the two microsatellite loci DRBPI (MHCII) and BOBT24 (IL4) were positively associated with disease susceptibility, demonstrating a corrected P-value of 0.002 and 0.005, respectively. In a second investigation, we tested 36 goats, naturally infected with the nematode parasite Trichostrongylus colubriformis. These animals were divided into a "low" and "high" excreting group on the basis of two independently recorded fecal egg counts. For this nematode resistance study, we detected a significant association of one of the alleles of the microsatellite locus SPS113 with "low" excretion (resistance). The MHC class II locus DYA (P19), was weakly associated with susceptibility in both diseases (Pc = 0.05). In future experiments, we will extend the sample size in order to verify the described associations. (Résumé d'auteur)

Mots-clés Agrovoc : Maladie infectieuse, Chèvre, Microsatellite, Génétique moléculaire, Résistance aux maladies

Mots-clés complémentaires : Gène de résistance

Classification Agris : L10 - Animal genetics and breeding

Auteurs et affiliations

  • Obexer-Ruff Gabriela, Institute of animal genetics (CHE)
  • Sattler Ursula, Institute of animal genetics (CHE)
  • Martinez Dominique, CIRAD-EMVT-SANTE ANIMALE (GLP)
  • Maillard Jean-Charles, CIRAD-EMVT-SANTE ANIMALE (FRA)
  • Chartier Christophe, AFSSA (FRA)
  • Saitbekova Nasikat, Institute of animal genetics (CHE)
  • Glowatzki Marie-Louise, Institute of animal genetics (CHE)
  • Gaillard Claude, Institute of animal genetics (CHE)

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