Humoral and cell-mediated immune responses of d/d histocompatible pigs against classical swine fever (CSF) virus

Piriou Laurence, Chevallier Sylvie, Hutet Evelyne, Charley Bernard, Le Potier Marie-Frédérique, Albina Emmanuel. 2003. Humoral and cell-mediated immune responses of d/d histocompatible pigs against classical swine fever (CSF) virus. Veterinary Research, 34 : 389-404.

Résumé : A better understanding of cell-mediated immune responses to classical swine fever virus (CSFV) is essential for the future development of improved vaccines. We analyzed the generation of cell-mediated and humoral immune responses in d/d histocompatible pigs following CSFV infection or vaccination. Viral infection induced high T cell responses with high primary and secondary CTL activity correlated with high IFN-[gamma] production, whereas vaccination with a live vaccine followed by infection mainly induced neutralizing antibody but low cell-mediated responses. Moreover, high IgG1 response was associated with high IFN-[gamma] response following infection whereas a weak IFN-[gamma] response was related to a good IgG2 response but a low IgG1 production. These data could reflect Th1/Th2-like balance of immune responses depending upon immunization protocols, which has not yet been described in the pig. T-cell responses to CSFV were evidenced by CSFV-specific CD25 upregulation on CD4-CD8+, but not on CD4+CD8- cells, which further illustrated the importance of CTL responses after infection. Our results indicated that generation of cell-mediated immune responses was much higher following intranasal/oral CSFV infection than after intramuscular vaccination, which implies that the capacity of new CSFV vaccines to induce higher T-cell responses should be considered.

Mots-clés Agrovoc : virus peste porcine, maladie des animaux, vaccin, toxicité, porcin, réponse immunitaire

Classification Agris : L50 - Physiologie et biochimie animales

Auteurs et affiliations

• Piriou Laurence, AFSSA (FRA)
• Chevallier Sylvie, CIRAD-FORET-FORETS NATURELLES (FRA)
• Hutet Evelyne, AFSSA (FRA)
• Charley Bernard, INRA (FRA)
• Le Potier Marie-Frédérique, AFSSA (FRA)
• Albina Emmanuel, CIRAD-EMVT-SANTE ANIMALE (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/515488/)

Métriques

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