Genetic and physical mapping of resistance and defence gene analogs colocalised with QTL of resistance for Phytophthora in cocoa

Lanaud Claire, Clément Didier, Fouet Olivier, Ruiz Manuel, Le Cunff Loïc, Risterucci Ange-Marie, Sabau Xavier, Glaszmann Jean-Christophe, Piffanelli Pietro. 2003. Genetic and physical mapping of resistance and defence gene analogs colocalised with QTL of resistance for Phytophthora in cocoa. In : 14th International Cocoa Research Conference, 13-18 October 2003, Accra, Ghana. Montpellier : Cirad, 1 p. Conférence Internationale sur la Recherche Cacaoyère. 14, Accra, Ghana, 13 Octobre 2003/18 Octobre 2003.

Communication par affiche

Prévisualisation

Version publiée - Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_516130.pdf
Télécharger (359kB) | Prévisualisation

Autre titre : Cartographie génétique et physique d'analogues de gènes de résistance et de défense co-localisés avec des QTL de résistance pour le Phytophthora chez le cacaoyer

Résumé : Resistance and defence gene analogs (RGA and DGA) have been isolated in cocoa using a PCR-based approach. Among 36 cocoa DNA fragments homologous to known resistance or defence genes, 16 corresponding to unique RFLP profiles could he mapped. One cluster of DGA and three clusters of RGA have been identified in the cocoa genome. One cluster corresponded to 2 PR2 like sequences, two clusters corresponded to genes encoding cytoplasmic receptor-like protein containing a nucleotide binding site (NBS). The third RGA cluster gathered four analogs to the tomato #Pto# gene and one analog to the #LRK10# wheat rust resistance gene, corresponding to genes which contain a serine threonine kinase domain (STK). This last cluster colocalised with 4 QTLs of resistance to Phytophthora identified in previous QTL mapping studies. A BAC library was constructed from the Scavina 6 resistant genotype and used to begin physical mapping of the chromosome regions containing these RGA/DGA clusters.

Mots-clés Agrovoc : Theobroma cacao, résistance aux maladies, Phytophthora, carte génétique, marqueur génétique

Mots-clés complémentaires : QTL

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Auteurs et affiliations