Garlic (Allium sativum L.) modulates cytokine expression in lipopolysaccharide-actived human blood thereby inhibiting NF-kB activity

Keiss Hans-Peter, Dirsch Verena M., Hartung Thomas, Haffner Thomas, Trueman Laurence, Auger Jacques, Kahane Rémi, Vollmar Angelika M.. 2003. Garlic (Allium sativum L.) modulates cytokine expression in lipopolysaccharide-actived human blood thereby inhibiting NF-kB activity. Journal of Nutrition, 133 (7) : pp. 2171-2175.

Journal article ; Article de revue à facteur d'impact
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Abstract : Garlic is proposed to have immunomodulatory and anti-inflammatory properties. This paper shows that garlic powder extracts (GPE) and single garlic metabolites modulate lipopolysaccharide (LPS)-induced cytokine levels in human whole blood. GPE-altered cytokine levels in human blood sample supernatants reduced nuclear factor (NF)-kB activity in human cells exposed to these samples. Pretreatment with GPE (100 mg/L) reduced LPS-induced production of proinflammatory cytokines interleukin (IL-1 [bêta] from 15.7 ± 5.1 to 6.2 ± 1.2 [mu]g/L and tumor necrosis factor (TNF)-[alpha] from 8.8 ± 2.4 to 3.9 ± 0.8 [mu]g/L, respectively, whereas the expression of the anti-inflammatory cytokine IL-10 was unchanged. The garlic metabolite diallydisulfide (1-100 [mu]mol/L) also significantly reduced IL-1[bêta] and TNF-[alpha]. Interestingly, exposure of human embryonic kidney cell line (HEK293) cells to GPE-treated blood sample supernatants (10 or 100 mg/L) reduced NF-K13 activity compared with cells exposed to untreated blood supernatants as measured by a NF-kB-driven luciferase reporter gene assay. Blood samples treated with extract obtained from unfertilized garlic (100 mg1L) reduced NF-kB activity by 25%, whereas blood samples treated with sulfur-fertilized garlic extracts (100 mg/L) lowered NF-kB activity by 41%. In summary, garlic may indeed promote an anti-inflammatory environment by cytokine modulation in human blood that leads to an overall inhibition of NF-kB activity in the surrounding tissue. (Résumé d'auteur)

Mots-clés Agrovoc : Allium sativum, Ail, Cytokine, Immunologie, Propriété pharmacologique, Sang, Genre humain

Classification Agris : Q04 - Food composition
S01 - Human nutrition - General aspects

Auteurs et affiliations

  • Keiss Hans-Peter, Ludwig-Maximilians-Universität (DEU)
  • Dirsch Verena M., Ludwig-Maximilians-Universität (DEU)
  • Hartung Thomas, Universität Konstanz (DEU)
  • Haffner Thomas, Lichtwer Pharma AG (DEU)
  • Trueman Laurence, COAGROCAUCA (COL)
  • Auger Jacques, Université François Rabelais (FRA)
  • Kahane Rémi, CIRAD-FLHOR-PMH (FRA) ORCID: 0000-0001-5492-2834
  • Vollmar Angelika M., Ludwig-Maximilians-Universität (DEU)

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