Myrie Wayne, Dollet Michel, McLauglin W., Been Basil.
2003. Study on the diversity of phytoplasma associated with lethal yellowing.
In : Pan American Plant disease Conference : 42nd Annual meeting on the American Phytopapthological Society-Caribbean Division, South Padre Island, Texas, U.S.A, April 6-10, 2003
Résumé : In the 70s, coconut lethal yellowing (LY) killed 10 million of local Talls; Malayan Dwarf and Maypan hybrid were thought to be "resistant". At the end of the 80s severe losses were recorded on these varieties. At the beginning of the 2000s, Maypan populations were destroyed. The LY agent was identified by PCR amplifying rRNA -primers P1/ P7- and non- ribosomal sequences - LYR1/LYF1. To test the hypothesis that this change could be due to changes in the pathogen, a study was undertaken on phytoplasmas variability. As there are no cultures or DNA stocks from the 70s, comparison was made between the phytoplasmas involved in the recent spread in Maypan, and those associated in local varieties in regions where no change has been observed. Study started with the comparison of the 16S/23S spacer region. The size of the products was compared, the RFLP, and some partial sequences of the amplified products were aligned and compared with other published LY sequences. (Texte intégral)
Mots-clés Agrovoc : Cocos nucifera, variété, phytoplasme, séquence nucléotidique
Classification Agris : H20 - Maladies des plantes
Auteurs et affiliations
- Myrie Wayne, Coconut Industry Board (JAM)
- Dollet Michel, CIRAD-EMVT-SANTE ANIMALE (FRA)
- McLauglin W., University of the West Indies (JAM)
- Been Basil, Coconut Industry Board (JAM)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/517444/)
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