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Detection of the African swine fever genomic DNA on dried pig blood filter paper

Guy-Gonzague M., Roger François, Rousset Dominique, Randriamparany Tantely, Crucière Catherine. 2003. Detection of the African swine fever genomic DNA on dried pig blood filter paper. Journal of Applied Research in Veterinary Medicine, 1 (2) : pp. 143-147.

Journal article ; Article de revue à comité de lecture
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Abstract : Polymerase chain reaction (PCR) is one of the best techniques to detect hemadsorbing, non-hemadsorbing, and/or noncytopathic African swine fever (ASF) strains. In some circumstances, the collection, storage, and transport of biologic samples to an appropriate laboratory are not easy. For this purpose, the blood sampling on filter paper, a method widely used for the research of genomes by PCR, might be useful. This study has shown that ASFV DNA remains detectable by PCR after dry storage of blood on filter paper for at least 3 months at 30°C. Thus, the PCR was applied to filter paper blood samples from 62 pigs collected in Malagasy areas infected by ASFV The ASFV DNA was amplified for 34 of 62 dried blood samples. The results were similar to the previous ones obtained with fresh samples collected at the beginning of the epizootic. (Résumé d'auteur)

Mots-clés Agrovoc : ADN, PCR, Virose, Peste porcine africaine, épidémie

Classification Agris : L73 - Animal diseases

Auteurs et affiliations

  • Guy-Gonzague M., AFSSA (FRA)
  • Roger François, CIRAD-EMVT-SANTE ANIMALE (FRA) ORCID: 0000-0002-1573-6833
  • Rousset Dominique, Institut Pasteur de Madagascar (MDG)
  • Randriamparany Tantely, Institut Pasteur de Madagascar (MDG)
  • Crucière Catherine, AFSSA (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/517968/)

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