Spatial control of transgene expression in rice (Oryza sativa L.) using the GAL4 enhancer trapping system

Johnson Alexander, Hibberd Jullian, Gay Céline, Essah Pauline A., Haseloff Jim, Tester Mark, Guiderdoni Emmanuel. 2005. Spatial control of transgene expression in rice (Oryza sativa L.) using the GAL4 enhancer trapping system. Plant Journal, 41 (5) : 779-789.

Résumé : We used enhancer trapping with the GAL4 transcriptional activator from yeast to obtain spatial control of transgene expression in all organs of the model monocotyledonous species rice (oryza sativa L. cv. Nipponbare). Our T-DNA enhancer trapping cassette consisted of two principle components: (1) the minimal promoter-equipped gal4 gene placed adjacent to the right border, and (2) the green fluorescent protein gene (gfp) fused to the upstream activation sequence element (UAS) to which GAL4 binds and activates expression, so that gfp expression corresponds to gal4 expression. Agrobacterium-mediated integration of the cassette into the rice genome often brings the gal4 gene under transcriptional control of local genomic enhancers and promoters, resulting in gal4/gfp expression patterns ranging in specificity from single-cell types to constitutive expression. We produced more than 13 000 enhancer trap lines with this cassette and screened To adult plants (1982 lines), T1 seed 12684 lines) and T, seedlings (2667 lines) for gfp expression. Approximately 30% of the lines produced GFP, and we identified lines with gfp expression in specific cell types of all major organs of the rice plant. Subsequently, using the GUS reporter gene (uidA), we demonstrated that UAS:geneXconstructs can be transactivated in specific cell types where gal4 and gfp are expressed, thus providing an excellent system for the manipulation of gene expression and physiological function in specific cell types of rice.

Mots-clés Agrovoc : expression des gènes, Oryza sativa, Monocotylédone, génie génétique, transfert de gène, séquence nucléotidique, lignée

Mots-clés complémentaires : Piégeage de séquences régulatrices, Tissu, Promoteur, Amplification de gènes

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Johnson Alexander, University of Cambridge (GBR)
• Hibberd Jullian, University of Cambridge (GBR)
• Gay Céline, CIRAD-AMIS-BIOTROP (FRA)
• Essah Pauline A., University of Cambridge (GBR)
• Haseloff Jim, University of Cambridge (GBR)
• Tester Mark, University of Cambridge (GBR)
• Guiderdoni Emmanuel, CIRAD-AMIS-BIOTROP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/525269/)

Métriques

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