AFLP and SSR polymorphism in a Coffea interspecific backcross progeny ((Coffea heterocalyx x C. canephora) x C. canephora)

Hamon Perla, Coulibaly Issa, Revol Benoît, Noirot Michel, Poncet Valérie, Lorieux Mathias, Carasco-Lacombe Catherine, Minier Jérôme, Dufour Magali, Hamon Serge. 2005. AFLP and SSR polymorphism in a Coffea interspecific backcross progeny ((Coffea heterocalyx x C. canephora) x C. canephora). In : 20th International Conference on Coffee Science, 11-15 October 2004, Bangalore, India = 20ème Colloque Scientifique International sur le Café ; 20. Internationales Wissenshaftliches Kolloquium über Kaffee ; 20e Coloquio Cientifico Internacional sobre el Café. ASIC. Paris : ASIC, pp. 699-701. ISBN 2-900212-19-7 Colloque Scientifique International sur le Café. 20, Bangalore, Inde, 11 October 2004/15 October 2004.

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Abstract : An interspecific cross (BC 1) involving a species with one of the biggest genomes in Coffea genus (C. heterocalyx (HET), qDNA = 1.74 pg) and a species with a medium genome size (C. canephora (CAN), qDNA = 1.43 pg) was studied using two types of molecular markers, AFLPTM and SSR. One hundred and eighty eight AFLP bands and 34 SSR primer pairs were suitable for the mapping. The total map length was 1360 cM with 190 loci distributed on 15 linkage groups. The results were compared to the results obtained previously on an interspecific BC 1 progeny involving a species with a medium size genome (C. liberica var. dewevrei, DEW) and a species with one of the smallest size genome (C. pseudozanguebariae, PSE). They are discussed considering three main points: 1) the interest of the different markers type, 2) - the genomic distribution of AFLPs and SSR markers and 3) - the relation between AFLP polymorphism and the genome size. (Résumé d'auteur)

Mots-clés Agrovoc : Coffea canephora, Coffea, Polymorphisme génétique, Hybridation interspécifique, Marqueur génétique, Génome, Dimension, Coffea liberica

Mots-clés complémentaires : SSR, Coffea pseudozanguebariae, Coffea heterocalyx, AFLP

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

  • Hamon Perla, CIRAD-CP-UMR DGPC (FRA)
  • Coulibaly Issa, CIRAD-CP-UMR DGPC (FRA)
  • Revol Benoît, CIRAD-CP-UMR DGPC (FRA)
  • Noirot Michel, CIRAD-CP-UMR DGPC (FRA)
  • Poncet Valérie, CIRAD-CP-UMR DGPC (FRA)
  • Lorieux Mathias, CNRS (COL)
  • Carasco-Lacombe Catherine, CIRAD-AMIS-BIOTROP (FRA)
  • Minier Jérôme, CIRAD-CP-UMR DGPC (FRA)
  • Dufour Magali, CIRAD-CP-CAFE (FRA)
  • Hamon Serge, IRD (FRA)

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