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Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants

Blanc Géraldine, Baptiste Christelle, Oliver Gérald, Martin Florence, Montoro Pascal. 2006. Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants. Plant Cell Reports, 24 (12) : pp. 724-733.

Journal article ; Article de revue à facteur d'impact
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Abstract : An efficient procedure for producing transgenic Hevea brasiliensis callus and plant lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA 105 harbouring the vector pCAMB1A230I . Transformation capacity and competence of the embryo-genic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20°C, the duration of this phase could be increased up to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryo-genesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1, 2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged. (Résumé d'auteur)

Mots-clés Agrovoc : Hevea brasiliensis, Agrobacterium tumefaciens, Transformation génétique, Embryogénèse somatique, Antibiotique, Plante transgénique, Séquence nucléotidique, ADN, Génie génétique, Cryoconservation

Mots-clés complémentaires : Bêta-glucuronidase, GUS

Classification Agris : F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Blanc Géraldine
  • Baptiste Christelle
  • Oliver Gérald, CIRAD-CP-UMR BEPC (FRA)
  • Martin Florence, CIRAD-CP-UMR BEPC (FRA)
  • Montoro Pascal, INRA (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/530928/)

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