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Cryopreservation of plant germplasm using the encapsulation-dehydration technique : review and case study on sugarcane

Gonzalez Arnao Maria Teresa, Engelmann Florent. 2006. Cryopreservation of plant germplasm using the encapsulation-dehydration technique : review and case study on sugarcane. CryoLetters, 27 (3) : 155-168.

Article de revue ; Article de revue à facteur d'impact
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Résumé : Encapsulation-dehydration is a cryopreservation technique based on the technology developed for producing synthetic seeds, i.e. the encapsulation of expiants in calcium alginate beads. Encapsulated expiants are then precultured in liquid medium with a high sucrose concentration and partially desiccated before freezing. Encapsulating the expiants allowsthe subsequent application of very drastic treatments including preculture with high sucrose concentrations and desiccation to low moisture contents which would be higly damaging or lethal to non-encapsulated samples. An encapsulation-dehydration protocol comprises the following steps: pretreatment, encapsulation, preculture, desiccation, freezing and storage, thawing and regrowth. Encapsulation-dehydration has been applied to around 40 different plant species. The optimization of the successive steps of the encapsulation-dehydration protocol is illustrated for sugarcane apices. (Résumé d'auteur)

Mots-clés Agrovoc : Congélation, Ressource génétique, Encapsulation, Déshydratation, Canne à sucre, Saccharum, Conservation du matériel génétique, Multiplication végétative

Classification Agris : F30 - Génétique et amélioration des plantes
F02 - Multiplication végétative des plantes

Axe stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Gonzalez Arnao Maria Teresa, Universidad Veracruzana (MEX)
  • Engelmann Florent, CIRAD-FLHOR-UPR Multiplication végétative (GLP)

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Source : Cirad - Agritrop

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