Identification of a tryptophan-like epitope borne by the variable surface glycoprotein (VSG) of African trypanosomes

Semballa Silla, Okomo-Assoumou M.C., Holzmuller Philippe, Büscher Philippe, Magez S., Lemesre J.L., Daulouède Sylvie, Courtois Pierrette, Geffard M., Vincendeau Philippe. 2007. Identification of a tryptophan-like epitope borne by the variable surface glycoprotein (VSG) of African trypanosomes. Experimental Parasitology, 115 (2) : pp. 173-180.

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Abstract : Antibodies (Ab) directed against a tryptophan-like epitope (WE) were previously detected in patients with human African trypanosomiasis (HAT). We investigated whether or not these Ab resulted from immunization against trypanosome antigen(s) expressing a WE. By Western blotting, we identified an antigen having an apparent molecular weight ranging from 60 to 65 kDa, recognized by purified rabbit anti-WE Ab. This antigen, present in trypomastigote forms, was absent in procyclic forms and Trypanosoma cruzi trypomastigotes. Using purified variable surface glycoproteins (VSG) from various trypanosomes, we showed that VSG was the parasite antigen recognized by these rabbit Ab. Anti-WE and anti-VSG Ab were purified from HAT sera by affinity chromatography. Immunoreactivity of purified antibodies eluted from affinity columns and of depleted fractions showed that WE was one of the epitopes borne by VSG. These data underline the existence of an invariant WE in the structure of VSG from several species of African trypanosomes. © 2006 Elsevier Inc. All rights reserved.

Mots-clés Agrovoc : Trypanosomose, Trypanosoma, Identification, Épidémiologie, Immunologie, Glycoprotéine, Tryptophane, Anticorps, Antigène

Mots-clés géographiques Agrovoc : Afrique

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Semballa Silla, Université de Bordeaux II (FRA)
  • Okomo-Assoumou M.C., Université de Bordeaux II (FRA)
  • Holzmuller Philippe, CIRAD-EMVT-UMR TRYPANOSOMES (FRA) ORCID: 0000-0002-8919-9081
  • Büscher Philippe, Institute of tropical medecine (BEL)
  • Magez S., EPHE (FRA)
  • Lemesre J.L., IRD (FRA)
  • Daulouède Sylvie, Université de Bordeaux II (FRA)
  • Courtois Pierrette, Université de Bordeaux II (FRA)
  • Geffard M., EPHE (FRA)
  • Vincendeau Philippe, Université de Bordeaux II (FRA)

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